Baba and I paid a visit to Air Liquid our helium supplier to confirm the purity of the helium sent to us. After a very lengthy discussing I found that it is currently grade 4.5 or 99.995%.
We wanted to make the Performance Reference Compound spike today but it did not happen. There were many factors delaying this. The main problem was the purity of hexanes we were going to use was in question. After doing an air injection on the gc which turned out clean shown below in first picture. I suspect the contamination from yesterday came from the hexanes (VWR brand new bottle) not the GC itself. This was confirmed by injection a different brand of hexanes (Panreac) shown below in second picture. It shows much smaller peaks (same scale on left) if you compare it the chromatogram from the previous day. We also injected another new bottle of hexanes (VWR) of the same lot and got similar results as yesterdays runs. Anna and I worked on most of this together.
There was not enough time in the day to make the standard and we will proceed tomorrow and if it checks out on the GC we will begin making LFT. If need be the CERES staff will be happy to work on Saturday to ensure the success of this project.Good news, we found a 10-100 ul pipet. This should aid us in making standards very much and these pipets seems to be of decent quality...brand name Hightech lab series LM.
We will try to pick up the "GC-care" package that glenn sent to CERES on friday morning.
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